The quantitative glucose and mineral nutrient requirements of mouse LS (suspension) cells in chemically defined medium.

The influences of glucose, phosphate, magnesium and potassium concentration on growth rate and maximum population density were studied. From the data obtained the growth yields for glucose and phosphorus were found to be 2-4x10* cells (i.e. 0-76 mg dry weight)/mg glucose and 2-2 x io (i.e. 69 /ig dry weight)//ig P. The growth response to magnesium and potassium was complex since there were threshold concentrations of these metals below which no growth occurred. The growth yield for potassium (2-4 x 10' cells (i.e. 75 fig dry weight) /figK) was obtained by measuring potassium uptake during growth. Omission of calcium ions from the medium resulted in poor reproducibility of growth.